STOmics-GeneExpression-S1 Workflow
Application Example: Visualizing Whole Mouse Embryos and Adult Mouse Brains Through Stereo-Seq
STOmics-GeneExpression-S1 Chips are patterned grids of probes containing spatial coordinates. Upon interaction with a tissue section, cDNA is synthesized in situ from mRNA captured by the chip probes. Sequencing the cDNA with their spatial coordinates allows in silico reconstitution of the spatial transcriptomic profile of the tissue section, allowing easy visualization and analysis.
STOmics-GeneExpression-S1 resolves the developmental structure-specific gene expression patterns in mouse embryos at centimeter-scale fields of view. A. Hematoxylin and eosin staining of an E16.5 embryo section. B. Unsupervised clustering analysis identifies different anatomical structures in the E16.5 embryo. C. Spatial expression heatmap of the indicated genes representing different developing structures in an E16.5 embryo section.
Recent Publications
Spatiotemporal Transcriptomic Atlas of Mouse Organogenesis Using DNA Nanoball Patterned Array
High-resolution Spatiotemporal Transcriptomic Maps of Developing Drosophila Embryos and Larvae
Spatiotemporal Mapping of Gene Expression Landscapes and Developmental Trajectories During Zebrafish Embryogenesis
Single-cell Stereo-seq Enables Cell Type-specific Spatial Transcriptome Characterization in Arabidopsis Leaves
Spatiotemporal Transcriptome at Single-cell Resolution Reveals Key Radial Glial Cell Population in Axolotl Telencephalon Development and Regeneration
Spatially-resolved Transcriptomics Analyses of Invasive Fronts in Solid Tumors